The role of the extracellular matrix in tumor cell metastasis. Stracke, M.L., Murata, J., Aznavoorian, S. Physiological mechanisms of tumor-cell invasion and migration. Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment. Tumor invasion and metastases: biochemical mechanisms. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. 'The 39 steps' in gene expression profiling: critical issues and proposed best practices for microarray experiments. Stromal therapy: the next step in ovarian cancer treatment. The microenvironment of the tumour-host interface. Proteomics of human breast ductal carcinoma in situ. Proteomic approaches to the diagnosis, treatment, and monitoring of cancer. Molecular portraits and the family tree of cancer.

Subjective differences in outcome are seen as a function of the immunohistochemical method used on a colorectal cancer tissue microarray. The total time required to carry out this protocol is typically 1–1.5 h. Herein we provide a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users.

A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, RNA transcript profiling, cDNA library generation, proteomics discovery and signal-pathway profiling. LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue microecology. Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future.

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